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Pcloneez-nrs-blunt-amp/hc cloning kit

SpletStrataClone Blunt PCR Cloning Kit 5 Map for the StrataClone Blunt PCR Cloning Vector pSC-B-amp/kan Feature Nucleotide Position β-galactosidase α-fragment coding sequence … Splet产品价格：. ￥1090.0. 零背景平端Blunt TOPO载体: 适用于pfu、KOD、Phusion等高保真聚合酶扩增制备的平末端片段的克隆. 零背景T载 TA TOPO载体: 适用于taq, LA-taq等聚合酶 …

CloneSmarter™ Zero Background Topo Blunt DNA Cloning Kit

SpletKit Configurations. The Zero Blunt PCR Cloning Kit is offered in a variety of configurations: with One Shot™ TOPO10 Chemically Competent E. coli (K2700-20 and K2700-40) and without competent cells (K2750-20 and K2750-40) in 20- and 40- reaction kit sizes. Fast & convenient: 5-minute, room-temperature ligation. Efficient: ccd B gene for ... SpletTOPO克隆试剂盒PcloneEZ-Blunt-TOPO-Cloning Kit C5861-25 货号：C5861-25 计量单位：盒 交货周期：现货 选择规格： 运杂费：登录后可查看运杂费 购买数量： 加入购物车 … church partner https://noagendaphotography.com

Zero Blunt™ PCR Cloning Kit - Thermo Fisher Scientific

SpletCloning plants is extremely useful in the mass production of seedlings for transplanting, and for the faithful reproduction of plants that don’t reproduce true from seed. Now that … SpletManual Vector Information MSDS COA. Inquire Now Buy Now Add to Cart Free Sample Avaliable. Product Details. pEASY® -Blunt Cloning Kit is designed for cloning and sequencing Pfu-amplified PCR products. •5 minutes fast ligation of Pfu-amplified PCR products. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white … SpletZero Blunt® PCRクローニングキットは、非組換え体によるバックグラウンドが低い条件下で、平滑末端のPCR断片（または、平滑末端のDNA断片）をクローニングできるようにデザインされています。 pCR®-Bluntベクターには、LacZαのC末端に融合した致死性の E. coli ccdB遺伝子が含まれています。 平滑末端PCR断片のライゲーションはlacZα- ccd B … dewey uth

TOPO克隆试剂盒PcloneEZ-Blunt-TOPO-Cloning Kit C5861-25

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Pcloneez-nrs-blunt-amp/hc cloning kit

SAFETY DATA SHEET - Agilent Technologies

Splet08. nov. 2024 · Choose a soil high in nitrogen, which helps clones thrive. Give your clones about 18 hours of weak light, like compact fluorescent light, each day for best results. … SpletPcloneEZ-Blunt TOPO Cloning Kit 载体抗性:Kan 作用：适用于pfu、KOD、Phusion等高保真聚合酶扩增制备的平末端片段的克隆。 TOPO克隆技术：1997年诞生的TOPO克隆技术， …

Pcloneez-nrs-blunt-amp/hc cloning kit

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SpletInvestigating Influenza Virus Polymerase Activity in Feline Cells Based on the Influenza Virus Minigenome Replication System Driven by the Feline SpletThe Agilent StrataClone PCR cloning kit enables high-efficiency, 5-minute cloning of PCR products at room temperature. The kits use the DNA rejoining activity of DNA …

SpletPCR fragments, synthetic DNA fragments as well as genomic DNA fragments obtained by mechanical shearing can all be effectively cloned. The cloning vector pCloneEZ-TA-Amp/HC and pCloneEZ-TA-Kan/HC included in this kit are high copy number plasmids engineered to tolerate mild toxic genes. SpletProduct Introduction. This product is the second-generation TOPO cloning kit, which contains the second-generation Topoisomerase, a vector containing the suicide gene ccdB, and a blunt-end factor. Using the second-generation Topoisomerase, with the most suitable buffer, it has higher activity and further improves the cloning efficiency.

SpletTOPO PCR Cloning Kit (Cat. no. 450245) is shipped with only the Zero Blunt ™TOPO PCR Cloning reagents (Box 1). Box Store at 1 −30°C to −10°C in a non-frost-free freezer 2 … SpletPcloneez Ta Amp Hc Cloning Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article …

SpletProduct Details. pEASY® -Blunt Simple Cloning Vector eliminates the multi-cloning sites of pEASY® -Blunt Cloning Vector. It is designed for cloning and sequencing Pfu-amplified PCR products. •5 minutes fast ligation of Pfu-amplified PCR products. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white selection.

SpletBlunt end cloning kits generally combine the blunt end insert with the linear vector and DNA ligase, which then joins the genetic components into a single, completed plasmid. Selection markers, such as the ccdB gene, are often present to allow positive selection of recombinant vectors. Blunting kits, which remove incompatible 5' or 3' DNA ... churchpartner.com promotional codeSpletStrataClone Blunt PCR Cloning Kit, Part Number 240207 化学品的推荐用途和限制用途 物质用途 :分析试剂。 240207-51StrataClone Blunt Vector Mix amp/kan 0.021 ml（毫升） (1 µl) 240207-53StrataClone Blunt Control Insert 0.01 ml（毫升） (50 ng 5 ng/µl) 240207-54StrataClone Blunt Cloning Buffer 0.063 ml（毫升） dewey veterinary hospitalSpletTA-TOPO 载体（Amp/Kan抗性）图谱： 不含酶切位点NRS ：C5853/Amp C5854/Kan 含酶切位点 ： C5863/AmpC5864/Kan Topo克隆真正零背景，载体不自连；再长片段都仅需连 … dewey veterinary clinic dewey ok